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Ann Clin Microbiol Antimicrob. 2015 Apr 12;14:23. doi: 10.1186/s12941-015-0079-z.

Simple multiplex PCR assays to detect common pathogens and associated genes encoding for acquired extended spectrum betalactamases (ESBL) or carbapenemases from surgical site specimens in Vietnam.

Author information

1
Department of Molecular Biology, Tran Hung Dao Hospital, No 1, Tran Hung Dao Street, Hai Ba Trung Dist, Hanoi, Vietnam. tatrungngo@gmail.com.
2
Department of Molecular Biology, Tran Hung Dao Hospital, No 1, Tran Hung Dao Street, Hai Ba Trung Dist, Hanoi, Vietnam. hiennguyen1810@yahoo.com.
3
Department of Molecular Biology, Tran Hung Dao Hospital, No 1, Tran Hung Dao Street, Hai Ba Trung Dist, Hanoi, Vietnam. tranthithanhhuyen@gmail.com.
4
Department of Molecular Biology, Tran Hung Dao Hospital, No 1, Tran Hung Dao Street, Hai Ba Trung Dist, Hanoi, Vietnam. thanhflame@gmail.com.
5
Department of Gastroenterology, Tran Hung Dao Hospital, No 1, Tran Hung Dao Street, Hai Ba Trung Dist, Hanoi, Vietnam. maibinhtieuhoa108@gmail.com.
6
Department of Molecular Biology, Tran Hung Dao Hospital, No 1, Tran Hung Dao Street, Hai Ba Trung Dist, Hanoi, Vietnam. phan_hoan2002@yahoo.com.
7
Institute of Tropical Medicine, University of Tuebingen, Tuebingen, Germany. c.g.meyer@bni.uni-hamburg.de.
8
Institute of Tropical Medicine, University of Tuebingen, Tuebingen, Germany. velavan@medizin.uni-tuebingen.de.
9
Fondation Congolaise pour la Recherche Medicale, Brazzaville, Republic of Congo. velavan@medizin.uni-tuebingen.de.
10
Department of Molecular Biology, Tran Hung Dao Hospital, No 1, Tran Hung Dao Street, Hai Ba Trung Dist, Hanoi, Vietnam. lehuusong@108-icid.com.
11
Institute of Clinical Infectious Diseases, Tran Hung Dao Hospital, No 1, Tran Hung Dao Street, Hai Ba Trung Dist, Hanoi, Vietnam. lehuusong@108-icid.com.

Abstract

Surgical site infection (SSI) is common in Vietnamese post-operative patients. It contributes to increased morbidity, mortality, hospitalization time and health care expenditure. Bacterial culture is considered the gold standard procedure to identify SSI pathogens and antibiotic resistant properties; however, it can detect microbes that can readily grow and is time-consuming. We propose optimized multiplex PCR assays to diagnose the most relevant microbes and associated genes encoding for acquired extended spectrum betalactamases (ESBL) or carbapenemases from Vietnamese patients with SSI in a hospital setting in Hanoi.

METHODS:

Ninety-one patients (nā€‰=ā€‰91) were collected in order to identify microbial pathogens and associated genes encoding for acquired extended spectrum betalactamases (ESBL) or carbapenemases by both conventional bacterial culture and in-house multiplex PCR assays.

RESULT AND CONCLUSION:

The novel in-house multiplex PCR assays are comparable to the bacterial culture approach in screening for common pathogens causing SSI and for relevant genotypes conferring betalactam/carbapenem resistance for bacteria. This is the first report of Turkey-specific ESBL gene (PER-1) and two Oxacilinase families (Oxa23 and Oxa 58) in Vietnam.

PMID:
25890291
PMCID:
PMC4399146
DOI:
10.1186/s12941-015-0079-z
[Indexed for MEDLINE]
Free PMC Article

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