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Daru. 2015 Feb 21;23:18. doi: 10.1186/s40199-015-0098-5.

Potential therapeutic effect of Allium cepa L. and quercetin in a murine model of Blomia tropicalis induced asthma.

Author information

1
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil. tati-oliveira@hotmail.com.
2
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil. kenia_maciely@hotmail.com.
3
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil. anaterezacl@gmail.com.
4
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil. tam_brasil@hotmail.com.
5
Faculdade de Farmácia, Universidade Federal da Bahia, Salvador, Bahia, Brazil. euvelozo@ufba.br.
6
Centro de Biotecnologia, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil. ingridcarm@hotmail.com.
7
Centro de Biotecnologia, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil. geninhaaf@gmail.com.
8
Centro de Biotecnologia, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil. eduardo@ltf.ufpb.br.
9
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil. darizy@gmail.com.
10
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil. carvalho@gmail.com.
11
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil. neuzalcantara@gmail.com.
12
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil. cavfigueiredo@gmail.com.

Abstract

BACKGROUND:

Asthma is an inflammatory condition characterized by airway hyperresponsiveness and chronic inflammation. The resolution of inflammation is an essential process to treat this condition. In this study we investigated the effect of Allium cepa L. extract (AcE) and quercetin (Qt) on cytokine and on smooth muscle contraction in vitro and its therapeutic potential in a murine model of asthma.

METHODS:

AcE was obtained by maceration of Allium cepa L. and it was standardized in terms of quercetin concentration using high performance liquid chromatography (HPLC). In vitro, using AcE 10, 100 or 1000 μg/ml or Qt 3.5, 7.5, 15 μg/ml, we measured the concentration of cytokines in spleen cell culture supernatants, and the ability to relax tracheal smooth muscle from A/J mice. In vivo, Blomia tropicalis (BT)-sensitized A/J mice were treated with AcE 100, 1000 mg/kg or 30 mg/kg Qt. We measured cell influx in bronchoalveolar lavage (BAL), eosinophil peroxidase (EPO) in lungs, serum levels of Bt-specific IgE, cytokines levels in BAL, and lung histology.

RESULTS:

We observed a reduction in the production of inflammatory cytokines, a relaxation of tracheal rings, and a reduction in total number of cells in BAL and EPO in lungs by treatment with AcE or Qt.

CONCLUSION:

AcE and Qt have potential as antiasthmatic drugs, as they possess both immunomodulatory and bronchodilatory properties.

PMID:
25890178
PMCID:
PMC4344790
DOI:
10.1186/s40199-015-0098-5
[Indexed for MEDLINE]
Free PMC Article

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