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BMC Microbiol. 2015 Apr 12;15:86. doi: 10.1186/s12866-015-0418-4.

Role of AcsR in expression of the acetyl-CoA synthetase gene in Vibrio vulnificus.

Author information

1
Department of Environmental Medical Biology and Institute of Tropical Medicine, Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, 120-752, South Korea. MJKIM55@yuhs.ac.
2
Department of Environmental Medical Biology and Institute of Tropical Medicine, Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, 120-752, South Korea. zhuri@yuhs.ac.
3
Department of Environmental Medical Biology and Institute of Tropical Medicine, Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, 120-752, South Korea. HYLEE0617@yuhs.ac.
4
Department of Environmental Medical Biology and Institute of Tropical Medicine, Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, 120-752, South Korea. no1081hj@naver.com.
5
Department of Life Science, Sogang University, Seoul, 121-742, South Korea. kyuholee@sogang.ac.kr.
6
Department of Environmental Medical Biology and Institute of Tropical Medicine, Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, 120-752, South Korea. sjpark615@yuhs.ac.

Abstract

BACKGROUND:

VarS/VarA is one of the global factors regulating diverse aspects of the metabolism and virulence of bacteria including pathogenic Vibrio spp. An experiment to identify the VarS/VarA-regulon in V. vulnificus revealed that a putative LuxR-type transcriptional regulator was down-regulated in ΔvarA mutant. To investigate the roles of this regulatory cascade, the target gene regulated by a LuxR-regulator was identified and its expression was characterized.

RESULTS:

Transcriptomic analysis of the mutant deficient in this LuxR-type regulator showed that the acsA gene encoding acetyl-CoA synthetase was down-regulated. Thus, this regulator was named AcsR for "regulator of acetyl-CoA synthetase". A putative histidine kinase gene, acsS, was located five ORFs downstream of the acsR gene. Expression of an acsA::luxAB transcriptional fusion was decreased in both ΔacsR and ΔacsS mutants. Similar to a ΔacsA mutant, strains carrying deletions either in acsR or acsS grew slowly than wild type in a minimal medium with acetate as a sole carbon source. Growth defect of the ΔacsR strain in acetate-minimal medium was restored by complementation. To investigate if AcsR directly regulates acsA expression, in vitro-gel shift assays were performed using the recombinant AcsR and the regulatory region of the acsA gene, showing that AcsR specifically bound the upstream region of the acsA ORF.

CONCLUSION:

This study indicates that the VarS/VarA system plays a role in V. vulnificus metabolism via regulating AcsR, which in turn controls acetate metabolism by activating the transcription of the acetyl-CoA synthetase gene.

PMID:
25887971
PMCID:
PMC4409781
DOI:
10.1186/s12866-015-0418-4
[Indexed for MEDLINE]
Free PMC Article

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