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Genome Biol. 2015 Apr 8;16:67. doi: 10.1186/s13059-015-0637-x.

Associations between host gene expression, the mucosal microbiome, and clinical outcome in the pelvic pouch of patients with inflammatory bowel disease.

Author information

1
Department of Biostatistics, Harvard T. H. Chan School of Public Health, 655 Huntington Ave, Boston, MA, 02115, USA. xmorgan@hsph.harvard.edu.
2
The Broad Institute of MIT and Harvard, 415 Main St, Cambridge, MA, 02142, USA. xmorgan@hsph.harvard.edu.
3
Mount Sinai Hospital, Zane Cohen Centre for Digestive Diseases, University of Toronto, 600 University Ave, Toronto, ON, M5G 1X5, Canada. btk2102@gmail.com.
4
Department of Biostatistics, Harvard T. H. Chan School of Public Health, 655 Huntington Ave, Boston, MA, 02115, USA. levi.waldron@hunter.cuny.edu.
5
City University of New York School of Public Health, Hunter College, 2180 3rd Ave Rm 538, New York, NY, 10035-4003, USA. levi.waldron@hunter.cuny.edu.
6
Mount Sinai Hospital, Zane Cohen Centre for Digestive Diseases, University of Toronto, 600 University Ave, Toronto, ON, M5G 1X5, Canada. atyler@mtsinai.on.ca.
7
Department of Biostatistics, Harvard T. H. Chan School of Public Health, 655 Huntington Ave, Boston, MA, 02115, USA. timothyltickle@gmail.com.
8
The Broad Institute of MIT and Harvard, 415 Main St, Cambridge, MA, 02142, USA. timothyltickle@gmail.com.
9
Mount Sinai Hospital, Zane Cohen Centre for Digestive Diseases, University of Toronto, 600 University Ave, Toronto, ON, M5G 1X5, Canada. rmilgrom@mtsinai.on.ca.
10
Mount Sinai Hospital, Zane Cohen Centre for Digestive Diseases, University of Toronto, 600 University Ave, Toronto, ON, M5G 1X5, Canada. jstempak@mtsinai.on.ca.
11
The Broad Institute of MIT and Harvard, 415 Main St, Cambridge, MA, 02142, USA. dgevers@broadinstitute.org.
12
The Broad Institute of MIT and Harvard, 415 Main St, Cambridge, MA, 02142, USA. xavier@molbio.mgh.harvard.edu.
13
Mount Sinai Hospital, Zane Cohen Centre for Digestive Diseases, University of Toronto, 600 University Ave, Toronto, ON, M5G 1X5, Canada. msilverberg@mtsinai.on.ca.
14
Department of Biostatistics, Harvard T. H. Chan School of Public Health, 655 Huntington Ave, Boston, MA, 02115, USA. chuttenh@hsph.harvard.edu.
15
The Broad Institute of MIT and Harvard, 415 Main St, Cambridge, MA, 02142, USA. chuttenh@hsph.harvard.edu.

Abstract

BACKGROUND:

Pouchitis is common after ileal pouch-anal anastomosis (IPAA) surgery for ulcerative colitis (UC). Similar to inflammatory bowel disease (IBD), both host genetics and the microbiota are implicated in its pathogenesis. We use the IPAA model of IBD to associate mucosal host gene expression with mucosal microbiomes and clinical outcomes. We analyze host transcriptomic data and 16S rRNA gene sequencing data from paired biopsies from IPAA patients with UC and familial adenomatous polyposis. To achieve power for a genome-wide microbiome-transcriptome association study, we use principal component analysis for transcript and clade reduction, and identify significant co-variation between clades and transcripts.

RESULTS:

Host transcripts co-vary primarily with biopsy location and inflammation, while microbes co-vary primarily with antibiotic use. Transcript-microbe associations are surprisingly modest, but the most strongly microbially-associated host transcript pattern is enriched for complement cascade genes and for the interleukin-12 pathway. Activation of these host processes is inversely correlated with Sutterella, Akkermansia, Bifidobacteria, and Roseburia abundance, and positively correlated with Escherichia abundance.

CONCLUSIONS:

This study quantifies the effects of inflammation, antibiotic use, and biopsy location upon the microbiome and host transcriptome during pouchitis. Understanding these effects is essential for basic biological insights as well as for well-designed and adequately-powered studies. Additionally, our study provides a method for profiling host-microbe interactions with appropriate statistical power using high-throughput sequencing, and suggests that cross-sectional changes in gut epithelial transcription are not a major component of the host-microbiome regulatory interface during pouchitis.

PMID:
25887922
PMCID:
PMC4414286
DOI:
10.1186/s13059-015-0637-x
[Indexed for MEDLINE]
Free PMC Article

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