Format

Send to

Choose Destination
BMC Genomics. 2015 Apr 12;16:290. doi: 10.1186/s12864-015-1459-7.

Modelling the genetic architecture of flowering time control in barley through nested association mapping.

Author information

1
Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle Wittenberg, Betty-Heimann-Str. 3, 06120, Halle, Germany.
2
Interdisciplinary Center for Crop Plant Research (IZN), Betty-Heimann-Str. 3, 06120, Halle, Germany.
3
Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Stadt Seeland, OT Gatersleben, Germany.
4
Current address: University of Dundee at the James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
5
Current address: Bayer CropScience NV, Technologiepark 38, 9052, Ghent, Belgium.
6
Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle Wittenberg, Betty-Heimann-Str. 3, 06120, Halle, Germany. klaus.pillen@landw.uni-halle.de.

Abstract

BACKGROUND:

Barley, globally the fourth most important cereal, provides food and beverages for humans and feed for animal husbandry. Maximizing grain yield under varying climate conditions largely depends on the optimal timing of flowering. Therefore, regulation of flowering time is of extraordinary importance to meet future food and feed demands. We developed the first barley nested association mapping (NAM) population, HEB-25, by crossing 25 wild barleys with one elite barley cultivar, and used it to dissect the genetic architecture of flowering time.

RESULTS:

Upon cultivation of 1,420 lines in multi-field trials and applying a genome-wide association study, eight major quantitative trait loci (QTL) were identified as main determinants to control flowering time in barley. These QTL accounted for 64% of the cross-validated proportion of explained genotypic variance (pG). The strongest single QTL effect corresponded to the known photoperiod response gene Ppd-H1. After sequencing the causative part of Ppd-H1, we differentiated twelve haplotypes in HEB-25, whereof the strongest exotic haplotype accelerated flowering time by 11 days compared to the elite barley haplotype. Applying a whole genome prediction model including main effects and epistatic interactions allowed predicting flowering time with an unmatched accuracy of 77% of cross-validated pG.

CONCLUSIONS:

The elaborated causal models represent a fundamental step to explain flowering time in barley. In addition, our study confirms that the exotic biodiversity present in HEB-25 is a valuable toolbox to dissect the genetic architecture of important agronomic traits and to replenish the elite barley breeding pool with favorable, trait-improving exotic alleles.

PMID:
25887319
PMCID:
PMC4426605
DOI:
10.1186/s12864-015-1459-7
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center