Format

Send to

Choose Destination
BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.

Gateway-compatible tissue-specific vectors for plant transformation.

Author information

1
Department of Biology, Washington University, St. Louis, MO, 63130, USA. martampaciorek@gmail.com.
2
Department of Biology, Washington University, St. Louis, MO, 63130, USA. fricke@wustl.edu.
3
Department of Biology, Washington University, St. Louis, MO, 63130, USA. strader@wustl.edu.

Abstract

BACKGROUND:

Understanding regulation of developmental events has increasingly required the use of tissue-specific expression of diverse genes affecting plant growth and environmental responses.

FINDINGS:

To allow for cloning of presumptive promoters with tissue-specific activities, we created two plant expression vectors with multiple cloning sites upstream of a Gateway cassette for expression of either untagged or YFP-tagged genes of interest. For fast and easy tissue-specific expression of desired genes, we further developed an initial set of Gateway-compatible tissue-specific gene expression vectors that allow for the expression of YFP-tagged or untagged proteins driven by the ALCOHOL DEHYDROGENASE1, CHLOROPHYLL A/B BINDING PROTEIN 1, COBRA LIKE1, EXPANSIN7, LATERAL ORGAN BOUNDARIES-DOMAIN 16, SCARECROW, UBIQUITIN10, and WOODEN LEG upstream regulatory regions.

CONCLUSIONS:

These vectors provide an invaluable resource to the plant community, allowing for rapid generation of a variety of tissue-specific expression constructs.

PMID:
25884475
PMCID:
PMC4352289
DOI:
10.1186/s13104-015-1010-6
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center