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J Struct Biol. 2015 Nov;192(2):146-50. doi: 10.1016/j.jsb.2015.04.002. Epub 2015 Apr 13.

MRC2014: Extensions to the MRC format header for electron cryo-microscopy and tomography.

Author information

1
National Resource for Automated Molecular Microscopy, Electron Microscopy Group, New York Structural Biology Center, 89 Convent Ave, New York, NY 10027, USA. Electronic address: acheng@nysbc.org.
2
MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, United Kingdom.
3
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA.
4
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 70030, USA.
5
FEI, Achsteweg Noord 5, 5600 KK Eindhoven, The Netherlands.
6
Univ Autonoma Madrid, Escuela Politécnia Superior, E-28049 Madrid, Spain.
7
National Resource for Automated Molecular Microscopy, Electron Microscopy Group, New York Structural Biology Center, 89 Convent Ave, New York, NY 10027, USA.
8
Howard Hughes Medical Institute and the Department of Biochemistry and Biophysics, University of California at San Francisco UCSF, MC 2240 600 16th Street, Room S412D, San Francisco, CA 94158-2517, USA.
9
Scientific Computing Department, STFC Daresbury Laboratory, Daresbury, Warrington WA4 4AD, United Kingdom. Electronic address: martyn.winn@stfc.ac.uk.

Abstract

The MRC binary file format is widely used in the three-dimensional electron microscopy field for storing image and volume data. Files contain a header which describes the kind of data held, together with other important metadata. In response to advances in electron microscopy techniques, a number of variants to the file format have emerged which contain useful additional data, but which limit interoperability between different software packages. Following extensive discussions, the authors, who represent leading software packages in the field, propose a set of extensions to the MRC format standard designed to accommodate these variants, while restoring interoperability. The MRC format is equivalent to the map format used in the CCP4 suite for macromolecular crystallography, and the proposal also maintains interoperability with crystallography software. This Technical Note describes the proposed extensions, and serves as a reference for the standard.

KEYWORDS:

Electron microscopy; File format; Image data; Macromolecular crystallography

PMID:
25882513
PMCID:
PMC4642651
DOI:
10.1016/j.jsb.2015.04.002
[Indexed for MEDLINE]
Free PMC Article

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