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BMC Endocr Disord. 2015 Apr 2;15:13. doi: 10.1186/s12902-015-0015-4.

Influence of phthalates on glucose homeostasis and atherosclerosis in hyperlipidemic mice.

Author information

1
Departments of Radiology & Medical Imaging and of Biochemistry & Molecular Genetics, University of Virginia, PO Box 801339, , 266 Snyder Bldg, 480 Ray C Hunt Drive, Charlottesville, VA, 22908, USA. wz4m@Virginia.EDU.
2
Department of Endocrinology of Jianghuai Hospital, Huaian, Jiangsu Province, 223001, China. wz4m@Virginia.EDU.
3
Departments of Radiology & Medical Imaging and of Biochemistry & Molecular Genetics, University of Virginia, PO Box 801339, , 266 Snyder Bldg, 480 Ray C Hunt Drive, Charlottesville, VA, 22908, USA. mc2xc@Virginia.EDU.
4
Departments of Radiology & Medical Imaging and of Biochemistry & Molecular Genetics, University of Virginia, PO Box 801339, , 266 Snyder Bldg, 480 Ray C Hunt Drive, Charlottesville, VA, 22908, USA. ws4v@virginia.edu.

Abstract

BACKGROUND:

Phthalates are widely used as plasticizer and are considered as a typical endocrine-disrupting chemical. Epidemiological studies have associated serum or urinary phthalate metabolites with the prevalence of type 2 diabetes or related phenotypes. However, direct evidence supporting a causal role for exposure to phthalates in type 2 diabetes is lacking.

METHODS:

To determine the potential influence of phthalates on glucose homeostasis and atherosclerosis, female apolipoprotein E-deficient (Apoe(-/-)) mice were started at 6 weeks of age on a Western diet together with or without Bis-(2-ethylhexyl) phthalate. Phthalate was administered in drinking water at a daily dosage of 100 mg/kg. We examined glucose and insulin tolerance, plasma glucose and triglyceride levels, body weight, and atherosclerotic lesions in the aortic root.

RESULTS:

Two weeks after treatment, phthalate-exposed mice had significantly higher fasting blood glucose level (97.9 ± 2.1 vs. 84.3 ± 5.3 mg/dl, P = 0.034) and exhibited a trend of increased glucose intolerance compared to control mice. Insulin tolerance test on non-fasted mice 3 weeks after treatment revealed that phthalate had little influence on insulin sensitivity though phthalate-treated mice had a higher glucose concentration (159.2 ± 6.0 vs. 145.2 ± 3.6 mg/dl; P = 0.086). On the Western diet, Apoe(-/-) mice showed a time-dependent rise in fasting plasma glucose and triglyceride levels. However, no significant differences were observed between phthalate-treated and control mice in either phenotype after 4, 8, and 12 weeks of phthalate exposure. Neither body weight nor atherosclerotic lesions of Apoe(-/-) mice was affected.

CONCLUSION:

This study indicates that exposure to phthalates gives rise to a brief interference of glucose homeostasis but has little impact on the development of type 2 diabetes and atherosclerosis in Apoe(-/-) mice.

PMID:
25881014
PMCID:
PMC4382848
DOI:
10.1186/s12902-015-0015-4
[Indexed for MEDLINE]
Free PMC Article

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