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Int J Nanomedicine. 2015 Apr 1;10:2635-46. doi: 10.2147/IJN.S77816. eCollection 2015.

Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution.

Author information

1
Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
2
Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; University Center for Energy Efficient Buildings (UCEEB), The Czech Technical University in Prague, Bustehrad, Czech Republic.
3
Department of Surgery, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
4
University Center for Energy Efficient Buildings (UCEEB), The Czech Technical University in Prague, Bustehrad, Czech Republic.
5
Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic.

Abstract

Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

KEYWORDS:

growth factors; hernia regeneration; in vitro; nanofibers; polypropylene mesh

PMID:
25878497
PMCID:
PMC4388102
DOI:
10.2147/IJN.S77816
[Indexed for MEDLINE]
Free PMC Article

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