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Nature. 2015 Apr 16;520(7547):307-11. doi: 10.1038/nature14397.

A resource for cell line authentication, annotation and quality control.

Author information

1
Department of Discovery Oncology, Genentech Inc., South San Francisco, California 94080, USA.
2
Department of Bioinformatics and Computational Biology, Genentech Inc., South San Francisco, California 94080, USA.
3
Department of Pathology, Genentech Inc., South San Francisco, California 94080, USA.

Abstract

Cell line misidentification, contamination and poor annotation affect scientific reproducibility. Here we outline simple measures to detect or avoid cross-contamination, present a framework for cell line annotation linked to short tandem repeat and single nucleotide polymorphism profiles, and provide a catalogue of synonymous cell lines. This resource will enable our community to eradicate the use of misidentified lines and generate credible cell-based data.

PMID:
25877200
DOI:
10.1038/nature14397
[Indexed for MEDLINE]

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