Abstract
Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated in HIV infection studies.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies / administration & dosage*
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B-Lymphocytes / metabolism
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Cells, Cultured
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Dendritic Cells / metabolism
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Dextrans / administration & dosage*
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Drug Delivery Systems / instrumentation*
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HIV / genetics
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HIV Infections / therapy
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HIV Infections / virology
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Humans
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Lab-On-A-Chip Devices*
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Mice
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Mice, Inbred C57BL
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RNA, Small Interfering / administration & dosage*
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RNA, Small Interfering / genetics
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RNAi Therapeutics
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T-Lymphocytes / metabolism
Substances
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Antibodies
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Dextrans
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RNA, Small Interfering