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Oxid Med Cell Longev. 2015;2015:204615. doi: 10.1155/2015/204615. Epub 2015 Mar 22.

Assessment of eccentric exercise-induced oxidative stress using oxidation-reduction potential markers.

Author information

1
Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou, 41221 Larissa, Greece.
2
Department of Exercise and Sport Sciences, University of Thessaly, 42100 Trikala, Greece.
3
Trauma Research Department, St. Anthony Hospital, Lakewood, CO 80228, USA ; Trauma Research Department, Swedish Medical Center, Englewood, CO 80113, USA ; Trauma Research Department, Medical Center of Plano, Plano, TX 75075, USA.

Abstract

The aim of the present study was to investigate the use of static (sORP) and capacity ORP (cORP) oxidation-reduction potential markers as measured by the RedoxSYS Diagnostic System in plasma, for assessing eccentric exercise-induced oxidative stress. Nineteen volunteers performed eccentric exercise with the knee extensors. Blood was collected before, immediately after exercise, and 24, 48, and 72 h after exercise. Moreover, common redox biomarkers were measured, which were protein carbonyls, thiobarbituric acid-reactive substances, total antioxidant capacity in plasma, and catalase activity and glutathione levels in erythrocytes. When the participants were examined as one group, there were not significant differences in any marker after exercise. However, in 11 participants there was a high increase in cORP after exercise, while in 8 participants there was a high decrease. Thus, the participants were divided in low cORP group exhibiting significant decrease in cORP after exercise and in high cORP group exhibiting significant increase. Moreover, only in the low cORP group there was a significant increase in lipid peroxidation after exercise suggesting induction of oxidative stress. The results suggested that high decreases in cORP values after exercise may indicate induction of oxidative stress by eccentric exercise, while high increases in cORP values after exercise may indicate no existence of oxidative stress.

PMID:
25874019
PMCID:
PMC4385679
DOI:
10.1155/2015/204615
[Indexed for MEDLINE]
Free PMC Article

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