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J Exp Bot. 2015 Jun;66(11):3189-99. doi: 10.1093/jxb/erv128. Epub 2015 Apr 6.

U-box E3 ubiquitin ligase PUB17 acts in the nucleus to promote specific immune pathways triggered by Phytophthora infestans.

Author information

1
Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK.
2
Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK.
3
Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK.
4
Durham Centre for Crop Improvement Technology School of Biological and Biomedical Sciences, Durham University, Durham DH1 3HP, UK.
5
Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070, China;
6
Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Paul.Birch@hutton.ac.uk tianzhd@mail.hzau.edu.cn.
7
Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Paul.Birch@hutton.ac.uk tianzhd@mail.hzau.edu.cn.

Abstract

Ubiquitination regulates many processes in plants, including immunity. The E3 ubiquitin ligase PUB17 is a positive regulator of programmed cell death (PCD) triggered by resistance proteins CF4/9 in tomato. Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here. Silencing StPUB17 in potato by RNAi and NbPUB17 in Nicotiana benthamiana by virus-induced gene silencing (VIGS) each enhanced P. infestans leaf colonization. PAMP-triggered immunity (PTI) transcriptional responses activated by flg22, and CF4/Avr4-mediated PCD were attenuated by silencing PUB17. However, silencing PUB17 did not compromise PCD triggered by P. infestans PAMP INF1, or co-expression of R3a/AVR3a, demonstrating that not all PTI- and PCD-associated responses require PUB17. PUB17 localizes to the plant nucleus and especially in the nucleolus. Transient over-expression of a dominant-negative StPUB17(V314I,V316I) mutant, which retained nucleolar localization, suppressed CF4-mediated cell death and enhanced P. infestans colonization. Exclusion of the StPUB17(V314I,V316I) mutant from the nucleus abolished its dominant-negative activity, demonstrating that StPUB17 functions in the nucleus. PUB17 is a positive regulator of immunity to late blight that acts in the nucleus to promote specific PTI and PCD pathways.

KEYWORDS:

Disease resistance; effector-triggered immunity; hypersensitive response; oomycete; plant defence; ubiquitination.

PMID:
25873665
PMCID:
PMC4449539
DOI:
10.1093/jxb/erv128
[Indexed for MEDLINE]
Free PMC Article

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