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Int J Mol Med. 2015 Jun;35(6):1683-9. doi: 10.3892/ijmm.2015.2169. Epub 2015 Apr 7.

LSm1 binds to the Dengue virus RNA 3' UTR and is a positive regulator of Dengue virus replication.

Author information

1
Department of Microbiology, Faculty of Preclinical Medicine, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
2
Department of Orthopaedics, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.

Abstract

Dengue virus (DENV) is a mosquito-transmitted flavivirus that can cause severe disease in humans. The DENV positive strand RNA genome contains 5' and 3' untranslated regions (UTRs) that have been shown to be required for virus replication and interaction with host cell proteins. In the present study LSm1 was identified as a host cellular protein involved in DENV RNA replication. By using two independent methodologies, we demonstrated a critical interaction between LSm1 and the 3' UTR of DENV. Furthermore, the confocal immunofluorescence analysis showed that the interaction between LSm1 and viral RNA is located in P-body around nucleoli in the cytoplasm. LSm1 knockdown by siRNA specifically reduced the levels of viral RNA in DENV-infected cells and infectious DENV particles in the supernatant. These results provide evidence that LSm1 binding to the DENV RNA 3' UTR positively regulates DENV RNA replication.

PMID:
25872476
DOI:
10.3892/ijmm.2015.2169
[Indexed for MEDLINE]

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