Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2015 Apr 28;112(17):E2139-48. doi: 10.1073/pnas.1423695112. Epub 2015 Apr 13.

Induced transcription and stability of CELF2 mRNA drives widespread alternative splicing during T-cell signaling.

Author information

1
Departments of Biochemistry and Biophysics and Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104; and.
2
Department of Cell and Molecular Medicine, University of California, San Diego, CA 92093.
3
Departments of Biochemistry and Biophysics and Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104; and klync@mail.med.upenn.edu.

Abstract

Studies in several cell types have highlighted dramatic and diverse changes in mRNA processing that occur upon cellular stimulation. However, the mechanisms and pathways that lead to regulated changes in mRNA processing remain poorly understood. Here we demonstrate that expression of the splicing factor CELF2 (CUGBP, Elav-like family member 2) is regulated in response to T-cell signaling through combined increases in transcription and mRNA stability. Transcriptional induction occurs within 6 h of stimulation and is dependent on activation of NF-κB. Subsequently, there is an increase in the stability of the CELF2 mRNA that correlates with a change in CELF2 3'UTR length and contributes to the total signal-induced enhancement of CELF2 expression. Importantly, we uncover dozens of splicing events in cultured T cells whose changes upon stimulation are dependent on CELF2 expression, and provide evidence that CELF2 controls a similar proportion of splicing events during human thymic T-cell development. Taken together, these findings expand the physiologic impact of CELF2 beyond that previously documented in developing neuronal and muscle cells to T-cell development and function, identify unappreciated instances of alternative splicing in the human thymus, and uncover novel mechanisms for CELF2 regulation that may broadly impact CELF2 expression across diverse cell types.

KEYWORDS:

CELF2; T cells; alternative splicing; mRNA stability; thymic development

PMID:
25870297
PMCID:
PMC4418860
DOI:
10.1073/pnas.1423695112
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center