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DNA Repair (Amst). 2015 Jun;30:38-45. doi: 10.1016/j.dnarep.2015.03.006. Epub 2015 Mar 25.

SWI/SNF recruitment to a DNA double-strand break by the NuA4 and Gcn5 histone acetyltransferases.

Author information

1
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606, USA.
2
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606, USA. Electronic address: Craig.Peterson@umassmed.edu.

Abstract

The DNA damage response to double-strand breaks (DSBs) is critical for cellular viability. Recent work has shown that a host of chromatin regulators are recruited to a DSB, and that they are important for the DNA damage response. However, the functional relationships between different chromatin regulators at DSBs remain unclear. Here we describe a conserved functional interaction among the chromatin remodeling enzyme, SWI/SNF, the NuA4 and Gcn5 histone acetyltransferases, and phosphorylation of histone H2A.X (γH2AX). Specifically, we find that the NuA4 and Gcn5 enzymes are both required for the robust recruitment of SWI/SNF to a DSB, which in turn promotes the phosphorylation of H2A.X.

KEYWORDS:

Chromatin; Gcn5; Histone acetyltransferase; Homologous recombination; NuA4; SWI/SNF

PMID:
25869823
PMCID:
PMC4425604
DOI:
10.1016/j.dnarep.2015.03.006
[Indexed for MEDLINE]
Free PMC Article

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