Format

Send to

Choose Destination
Vascul Pharmacol. 2015 Aug;71:93-101. doi: 10.1016/j.vph.2015.03.014. Epub 2015 Apr 11.

Infliximab improves endothelial dysfunction in a mouse model of antiphospholipid syndrome: Role of reduced oxidative stress.

Author information

1
Inserm U1096, Rouen, France; Rouen University Hospital, Department of Internal Medicine, Rouen France; University of Rouen, Institute for Research and Innovation in Biomedicine, Rouen, France. Electronic address: ygal.benhamou@chu-rouen.fr.
2
Inserm U1096, Rouen, France; Rouen University Hospital, Department of Internal Medicine, Rouen France; University of Rouen, Institute for Research and Innovation in Biomedicine, Rouen, France.
3
Inserm U1096, Rouen, France; University of Rouen, Institute for Research and Innovation in Biomedicine, Rouen, France.
4
University of Rouen, Institute for Research and Innovation in Biomedicine, Rouen, France; Inserm U905, Rouen, France.
5
Pharmacovigilance Unit, La Pitié-Salpétrière Hospital, AP-HP, Paris France.
6
Rouen University Hospital, Department of Internal Medicine, Rouen France; University of Rouen, Institute for Research and Innovation in Biomedicine, Rouen, France.

Abstract

Antiphospholipid syndrome (APS), induces endothelial dysfunction, oxidative stress and systemic inflammation that may be mediated by TNFα. Thus, we investigated the possible protective effect of the anti-TNFα antibody infliximab (5μg/g) on endothelial function in a mouse APS model (induced by injection of purified human anti-β2GP1-IgG). Seven days after anti-β2GPI-IgG injection, we observed an increase in plasma sVCAM-1 and sE-selectin levels and in aortic mRNA expression of VCAM-1 and E-selectin. This was associated with a decreased endothelium-dependent relaxation of isolated mesenteric arteries to acetylcholine, together with decreased mesenteric eNOS mRNA expression and increased eNOS uncoupling, accompanied by increased iNOS and gp91phox mRNA and increased left ventricular GSH/GSSH ratio. Infliximab significantly improved the NO-mediated relaxing responses to acetylcholine, and induced a decrease in iNOS and gp91phox mRNA expression. The õpro-adhesive and pro-coagulant phenotypes induced by the anti-β2GP1-IgG were also reversed. This study provides the first evidence that TNFα antagonism improves endothelial dysfunction in APS and suggests that endothelial dysfunction is mediated by TNFα and oxidative stress. Therefore, infliximab may be of special relevance in clinical practice.

KEYWORDS:

Antiphospholipid antibodies; Endothelium; Infliximab; Oxidative stress; Tumor necrosis factor alpha

PMID:
25869505
DOI:
10.1016/j.vph.2015.03.014
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center