Send to

Choose Destination
See comment in PubMed Commons below
Oncotarget. 2015 Apr 20;6(11):9099-112.

The SOX17/miR-371-5p/SOX2 axis inhibits EMT, stem cell properties and metastasis in colorectal cancer.

Li Y1,2, Lv Z1,3,4, He G1,2, Wang J1,2, Zhang X1,5, Lu G1,2, Ren X1,2, Wang F1,2, Zhu X1,2, Ding Y6, Liao W1,2, Ding Y1,2, Liang L1,2.

Author information

Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, People's Republic of China.
Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou, Guangdong Province, People's Republic of China.
Department of General Surgery Two, Nanchong Central Hospital, Nanchong city, Sichuan Province, People's Republic of China.
The Second Clinical School of Northern Sichuan Medical College, Nanchong City, Sichuan Province, People's Republic of China.
Department of Pathology, Shenzhen University, Shenzhen, People's Republic of China.
Department of Radiotherapy, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, People's Republic of China.


Cancer stem cells (CSCs) and EMT-type cells, which share molecular characteristics with CSCs, have been believed to play critical roles in tumor metastasis. Although much progress has been garnered in elucidating the molecular pathways that trigger EMT, stemness and metastasis, a number of key mechanistic gaps remain elusive. In the study, miR-371-5p was obviously down-regulated in primary CRC tissues compared with matched adjacent normal mucosa and correlated significantly with differentiation, tumor size, lymphatic and liver metastases. MiR-371-5p could attenuate proliferation, invasion in vitro and metastasis in vivo in CRC cells. It also suppressed EMT by regulating Wnt/β-catenin signaling and strongly decreased the CRC stemness phenotypes. Moreover, demethylation of SOX17 induced miR-371-5p expression and consequently suppressed its direct target SOX2 in CRC cells. MiR-371-5p was necessary for SOX17 mediated cancer-related traits and SOX2 was a functional target of miR-371-5p. A positive relationship between SOX17 and miR-371-5p expression and a negative one between miR-371-5p and SOX2 expression were observed in CRC cell lines and tissues. In conclusion, we identified miR-371-5p as an important "oncosuppressor" in CRC progression and elucidated a novel mechanism of the SOX17/miR-371-5p/SOX2 axis in the regulation of EMT, stemness and metastasis, which may be a potential therapeutic target.


SOX17; SOX2; colorectal cancer; metastasis; miR-371-5p

[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Impact Journals, LLC Icon for PubMed Central
    Loading ...
    Support Center