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Future Microbiol. 2015;10(4):471-87. doi: 10.2217/fmb.14.136.

Host-pathogen interactions in Vibrio vulnificus: responses of monocytes and vascular endothelial cells to live bacteria.

Author information

1
Estructura de Investigación Interdisciplinar en Biotecnología y Medicina (ERI BIOTECMED). Department of Microbiology & Ecology, University of Valencia, 46100 Burjassot, Valencia, Spain.

Abstract

AIM:

To demonstrate that Vibrio vulnificus, a sepsis-related aquatic pathogen, can provoke a strong pro-inflammatory reaction in blood-associated target cells.

MATERIALS & METHODS:

We selected two strains of the two main phylogenetic lineages, two human cell lines, monocytes and vascular endothelial cells and designed an in vitro infection model simulating early septicemia.

RESULTS:

Both strains caused a strong cell-specific pro-inflammatory response and produced a high degree of cell damage that ended with death by lysis (endothelial cells) or apoptosis/lysis (monocytes). The interaction with endothelial cells was stronger than expected and significantly different for both lineages.

CONCLUSION:

The early interaction with endothelial cells could have a direct role in sepsis and could explain, at least partially, the differences in pathogenicity between both lineages.

KEYWORDS:

Vibrio vulnificus; apoptosis; cytokines; human vibriosis; monocytes; pathogen recognition receptors; transcription factors; vascular endothelial cells

PMID:
25865188
DOI:
10.2217/fmb.14.136
[Indexed for MEDLINE]

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