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Eur J Nutr. 2016 Mar;55(2):749-758. doi: 10.1007/s00394-015-0895-0. Epub 2015 Apr 11.

Acute administration of single oral dose of grape seed polyphenols restores blood pressure in a rat model of metabolic syndrome: role of nitric oxide and prostacyclin.

Author information

1
Nutrigenomic Research Group, Biochemistry and Biotechnology Department, Universitat Rovira i Virgili, C/Marcel.lí Domingo, 43007, Tarragona, Spain.
2
Nutrigenomic Research Group, Biochemistry and Biotechnology Department, Universitat Rovira i Virgili, C/Marcel.lí Domingo, 43007, Tarragona, Spain. anna.arola@urv.cat.
3
Centre Tecnològic de Nutrició i Salut (CTNS), TECNIO, CEICS, Tarragona, Spain.

Abstract

PURPOSE:

The aims of this study were to evaluate the antihypertensive effectiveness of different doses of grape seed polyphenols in cafeteria diet-fed hypertensive rats (CHRs) and to establish the mechanism involved in the blood pressure (BP) lowering effect of these compounds in this experimental model of metabolic syndrome (MS).

METHODS:

Male 8-week-old Wistar rats were fed cafeteria or standard (ST) diet for 10 weeks. After this, the antihypertensive effect of a single oral administration of a polyphenol grape seed extract (GSPE) was tested at different doses (250, 375 and 500 mg/kg) in CHRs. BP was recorded before and 2, 4, 6, 8, 24 and 48 h post-administration. The hypotensive effect of GSPE was also proved in ST diet-fed rats. Additionally, in other experiment, CHRs were orally administered 375 mg/kg GSPE. Four hours post-administration, the rats were intraperitoneally administrated 30 mg/kg NG-nitro-L-arginine methyl ester (L-NAME) or 5 mg/kg indomethacin [inhibitors of nitric oxide (NO) and prostacyclin synthesis, respectively]. BP was recorded initially and 6 h post-administration.

RESULTS:

GSPE produced a decrease in SBP and DBP, the most effective dose (375 mg/kg) showing an antihypertensive effect in CHRs similar to the drug captopril, and did not affect BP of ST diet-fed rats. The antihypertensive effect was completely abolished by L-NAME and partially inhibited by indomethacin.

CONCLUSIONS:

GSPE acts as an antihypertensive agent in a rat model of hypertension associated with MS. The change in endothelium-derived NO availability is one of the mechanisms involved in the antihypertensive effect of GSPE in CHRs. Additionally, endothelial prostacyclin contributes to the effect of GSPE on arterial BP.

KEYWORDS:

Antihypertensive agent; Cafeteria diet; Endothelial-relaxing factors; Hypertension

PMID:
25862540
DOI:
10.1007/s00394-015-0895-0
[Indexed for MEDLINE]

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