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Cell. 2015 Apr 9;161(2):228-39. doi: 10.1016/j.cell.2015.03.026.

Ubiquitous L1 mosaicism in hippocampal neurons.

Author information

1
Mater Research Institute - University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia.
2
Department of Child Neurology, Neuroscience Campus Amsterdam, VU University Medical Center, 1081 HV Amsterdam, The Netherlands.
3
Edinburgh Cancer Research Centre, Western General Hospital, Edinburgh, EH4 2XR, UK.
4
Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC 20010, USA.
5
Mater Research Institute - University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia; Queensland Brain Institute, University of Queensland, Brisbane QLD 4072, Australia. Electronic address: faulknergj@gmail.com.

Abstract

Somatic LINE-1 (L1) retrotransposition during neurogenesis is a potential source of genotypic variation among neurons. As a neurogenic niche, the hippocampus supports pronounced L1 activity. However, the basal parameters and biological impact of L1-driven mosaicism remain unclear. Here, we performed single-cell retrotransposon capture sequencing (RC-seq) on individual human hippocampal neurons and glia, as well as cortical neurons. An estimated 13.7 somatic L1 insertions occurred per hippocampal neuron and carried the sequence hallmarks of target-primed reverse transcription. Notably, hippocampal neuron L1 insertions were specifically enriched in transcribed neuronal stem cell enhancers and hippocampus genes, increasing their probability of functional relevance. In addition, bias against intronic L1 insertions sense oriented relative to their host gene was observed, perhaps indicating moderate selection against this configuration in vivo. These experiments demonstrate pervasive L1 mosaicism at genomic loci expressed in hippocampal neurons.

PMID:
25860606
PMCID:
PMC4398972
DOI:
10.1016/j.cell.2015.03.026
[Indexed for MEDLINE]
Free PMC Article

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