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Ophthalmic Surg Lasers Imaging Retina. 2015 Mar;46(3):362-8. doi: 10.3928/23258160-20150323-11.

Serum inflammatory markers after rupture retinal laser injury in mice.

Abstract

BACKGROUND AND OBJECTIVE:

To characterize the cellular, immunological, and inflammatory response to retinal photocoagulation of intense rupture laser lesions as a model of retinal degenerative diseases.

MATERIALS AND METHODS:

Seven C57BL/6 mice were irradiated using a 532-nm laser to induce 10 retinal burns per eye that ruptured Bruch's membrane. Blood was drawn from the saphenous vein before and 2 months after laser treatment. The serum was run on antigen microarrays with 85 molecular markers associated with retinal degenerative diseases.

RESULTS:

Rupture laser resulted in dramatic changes in the immunoglobulin reactivity of most inflammatory markers 2 months after laser injury. Approximately two-thirds increased expression and one-third decreased expression. Notable markers that were increased included complement C3, CRP, PKM2, and aldolase.

CONCLUSION:

Rupture laser injury causes a change in the serum inflammatory markers after 2 months similar to macular degeneration, diabetic retinopathy, and cancer-associated retinopathy. This animal model could be used as a biomarker for disease stage and activity in retinal degenerations.

PMID:
25856824
DOI:
10.3928/23258160-20150323-11
[Indexed for MEDLINE]

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