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Scand J Clin Lab Invest. 2015 Sep;75(5):355-61. doi: 10.3109/00365513.2015.1030765. Epub 2015 Apr 9.

Validation of a new extraction device for measuring faecal calprotectin in inflammatory bowel disease, and comparison to established extraction methods.

Author information

1
Unger-Vetlesen's Institute, Department of Medicine, Lovisenberg Diakonale Hospital , Oslo , Norway.

Abstract

OBJECTIVE:

Faecal (f-) calprotectin is a widely used surrogate marker of intestinal inflammation. F-calprotectin analysis is labour demanding partly due to a time consuming extraction step. The aim of this project was to validate a new extraction method for f-calprotectin.

MATERIAL AND METHODS:

A prospective multicentre study included 135 patients with an established diagnosis of inflammatory bowel disease. The patients submitted a faeces sample, which was extracted with both the conventional method at the participating laboratory and the new extraction device. The extracts were analyzed by an automated ELISA instrument.

RESULTS:

Method comparison of the traditional method and the new device showed a slope of 1.01 (0.93-1.07) with intercept of - 2.2 (- 4.9-0.6). The Spearman rank correlation coefficient was 0.96.

CONCLUSIONS:

The new extraction device is a reliable and time saving alternative to the conventional extraction method.

KEYWORDS:

Biological markers; Crohn disease; colitis; enzyme-linked immunosorbent assay; faeces; inflammatory bowel disease; leukocyte L1 antigen complex; ulcerative

PMID:
25856663
DOI:
10.3109/00365513.2015.1030765
[Indexed for MEDLINE]

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