A single amino acid deletion in the matrix protein of porcine reproductive and respiratory syndrome virus confers resistance to a polyclonal swine antibody with broadly neutralizing activity

J Virol. 2015 Jun;89(12):6515-20. doi: 10.1128/JVI.03287-14. Epub 2015 Apr 8.

Abstract

Assessment of virus neutralization (VN) activity in 176 pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV) identified one pig with broadly neutralizing activity. A Tyr-10 deletion in the matrix protein provided escape from broad neutralization without affecting homologous neutralizing activity. The role of the Tyr-10 deletion was confirmed through an infectious clone with a Tyr-10 deletion. The results demonstrate differences in the properties and specificities of VN responses elicited during PRRSV infection.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies, Neutralizing / immunology*
  • Antibodies, Viral / immunology*
  • Molecular Sequence Data
  • Porcine respiratory and reproductive syndrome virus / immunology*
  • Sequence Analysis, DNA
  • Sequence Deletion*
  • Swine
  • Tyrosine / genetics*
  • Viral Matrix Proteins / genetics
  • Viral Matrix Proteins / immunology*

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • M protein, porcine reproductive and respiratory syndrome virus
  • Viral Matrix Proteins
  • Tyrosine

Associated data

  • GENBANK/KM035798