Format

Send to

Choose Destination
J Clin Microbiol. 2015 Jun;53(6):1908-14. doi: 10.1128/JCM.00025-15. Epub 2015 Apr 8.

PhyResSE: a Web Tool Delineating Mycobacterium tuberculosis Antibiotic Resistance and Lineage from Whole-Genome Sequencing Data.

Author information

1
Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany German Center for Infection Research (DZIF), Borstel Site, Borstel, Germany.
2
Bioinformatics, Research Center Borstel, Borstel, Germany.
3
Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany.
4
Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.
5
Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany German Center for Infection Research (DZIF), Borstel Site, Borstel, Germany sniemann@fz-borstel.de kfellenberg@fz-borstel.de.
6
Bioinformatics, Research Center Borstel, Borstel, Germany sniemann@fz-borstel.de kfellenberg@fz-borstel.de.

Abstract

Antibiotic-resistant tuberculosis poses a global threat, causing the deaths of hundreds of thousands of people annually. While whole-genome sequencing (WGS), with its unprecedented level of detail, promises to play an increasingly important role in diagnosis, data analysis is a daunting challenge. Here, we present a simple-to-use web service (free for academic use at http://phyresse.org). Delineating both lineage and resistance, it provides state-of-the-art methodology to life scientists and physicians untrained in bioinformatics. It combines elaborate data processing and quality control, as befits human diagnostics, with a treasure trove of validated resistance data collected from well-characterized samples in-house and worldwide.

PMID:
25854485
PMCID:
PMC4432036
DOI:
10.1128/JCM.00025-15
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center