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Nat Methods. 2015 May;12(5):423-5. doi: 10.1038/nmeth.3351. Epub 2015 Apr 6.

Sequencing small genomic targets with high efficiency and extreme accuracy.

Author information

1
1] Department of Medicine, Divisions of Hematology and Medical Oncology, University of Washington, Seattle, Washington, USA. [2] Department of Pathology, University of Washington, Seattle, Washington, USA. [3] Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
2
Department of Pathology, University of Washington, Seattle, Washington, USA.
3
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

Abstract

The detection of minority variants in mixed samples requires methods for enrichment and accurate sequencing of small genomic intervals. We describe an efficient approach based on sequential rounds of hybridization with biotinylated oligonucleotides that enables more than 1-million-fold enrichment of genomic regions of interest. In conjunction with error-correcting double-stranded molecular tags, our approach enables the quantification of mutations in individual DNA molecules.

PMID:
25849638
PMCID:
PMC4414912
DOI:
10.1038/nmeth.3351
[Indexed for MEDLINE]
Free PMC Article
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