Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2015 Apr 28;112(17):E2130-8. doi: 10.1073/pnas.1414242112. Epub 2015 Apr 6.

FtsZ filament capping by MciZ, a developmental regulator of bacterial division.

Author information

1
Departamento de Bioquímica, IQ, Universidade de São Paulo, 05508000, São Paulo, SP, Brazil;
2
Université Grenoble Alpes, Centre National de la Recherche Scientifique, and Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut de Biologie Structurale, F-38044 Grenoble, France;
3
Departamento de Bioquímica, IQ, Universidade de São Paulo, 05508000, São Paulo, SP, Brazil; Brazilian Biosciences National Laboratory, LNBio, Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), 13083-970, Campinas, SP, Brazil;
4
Brazilian Biosciences National Laboratory, LNBio, Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), 13083-970, Campinas, SP, Brazil;
5
Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, 09210580, Santo André, SP, Brazil; and.
6
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710-3709.
7
Université Grenoble Alpes, Centre National de la Recherche Scientifique, and Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut de Biologie Structurale, F-38044 Grenoble, France; Brazilian Biosciences National Laboratory, LNBio, Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), 13083-970, Campinas, SP, Brazil;
8
Departamento de Bioquímica, IQ, Universidade de São Paulo, 05508000, São Paulo, SP, Brazil; fgueiros@iq.usp.br.

Abstract

Cytoskeletal structures are dynamically remodeled with the aid of regulatory proteins. FtsZ (filamentation temperature-sensitive Z) is the bacterial homolog of tubulin that polymerizes into rings localized to cell-division sites, and the constriction of these rings drives cytokinesis. Here we investigate the mechanism by which the Bacillus subtilis cell-division inhibitor, MciZ (mother cell inhibitor of FtsZ), blocks assembly of FtsZ. The X-ray crystal structure reveals that MciZ binds to the C-terminal polymerization interface of FtsZ, the equivalent of the minus end of tubulin. Using in vivo and in vitro assays and microscopy, we show that MciZ, at substoichiometric levels to FtsZ, causes shortening of protofilaments and blocks the assembly of higher-order FtsZ structures. The findings demonstrate an unanticipated capping-based regulatory mechanism for FtsZ.

KEYWORDS:

FtsZ; bacterial cytoskeleton; cell division; cytokinesis; filament capping

PMID:
25848052
PMCID:
PMC4418908
DOI:
10.1073/pnas.1414242112
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center