Format

Send to

Choose Destination
World J Pediatr. 2016 May;12(2):177-82. doi: 10.1007/s12519-015-0025-3. Epub 2015 Apr 6.

Effects of Bifidobacterium supplementation on intestinal microbiota composition and the immune response in healthy infants.

Author information

1
Pediatrics Institute, Key Laboratory of Neonatal Diseases, Ministry of Health, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai, 201102, China.
2
Department of Pediatric Healthcare, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai, 201102, China.
3
Pediatrics Institute, Key Laboratory of Neonatal Diseases, Ministry of Health, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai, 201102, China. wpwang@fudan.edu.cn.

Abstract

BACKGROUND:

Intestinal microbiotas are thought to be the most important source of maturational stimuli to the development of the immune system. However, few studies have focused on the development of T helper (Th) 1 immune response and antibody response to vaccinations in healthy infants, especially in a large cohort. Through this randomized, double-blind control trial, we investigated the effects of Bifidobacterium longum BB536 (BB536) supplementation on intestinal microbiota composition and the immune response in term infants.

METHODS:

In total, 300 healthy newborns were recruited, randomized and fed formula either supplemented with BB536 or with no supplementation. Stool samples were analyzed at months 2, 4 and 11. The representative cytokine for Th1 [interferon-γ (IFN-γ)] and Th2 [interleukin-4 (IL-4)] secretion cells were measured using enzyme-linked immunospot assay at 4 and 7 months of age. The antibody response to vaccines was measured at months 7 and 11.

RESULTS:

A total of 264 infants completed the study. The amount of bifidobacteria and the bifidobacteria/ Enterobacteriaceae ratio (B/E) were significantly higher in the BB536 supplementation group at months 2 and 4. The number of IFN-γ secretion cells and the ratio of IFN-γ/IL-4 secretion cells were increased in the BB536 supplementation group at 7 months. Moreover, the higher value of B/E in the early stages seems to be related to the increased Th1 response. No difference was observed between groups in the antibody response after vaccination.

CONCLUSION:

BB536 has positive effects on establishing a healthy intestinal microbiota early in life, and it also plays an important role in improving the Th1 immune response.

KEYWORDS:

T helper 1/T helper 2 balance; intestinal microbiota; probiotics supplementation; term infants; vaccination

PMID:
25846071
DOI:
10.1007/s12519-015-0025-3
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center