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J Immunol Methods. 2015 Jun;421:89-95. doi: 10.1016/j.jim.2015.03.014. Epub 2015 Apr 2.

A 3-D enteroid-based model to study T-cell and epithelial cell interaction.

Author information

1
Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA.
2
Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA. Electronic address: mucida@rockefeller.edu.

Abstract

The constant interaction between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) is thought to regulate mucosal barrier function and immune responses against invading pathogens. IELs represent a heterogeneous population of mostly activated and antigen-experienced T cells, but the biological function of IELs and their relationship with IECs is still poorly understood. Here, we describe a method to study T-cell-epithelial cell interactions using a recently established long-term intestinal "enteroid" culture system. This system allowed the study of peripheral T cell survival, proliferation, differentiation and behavior during long-term co-cultures with crypt-derived 3-D enteroids. Peripheral T cells activated in the presence of enteroids acquire several features of IELs, including morphology, membrane markers and movement in the epithelial layer. This co-culture system may facilitate the investigation of complex interactions between intestinal epithelial cells and immune cells, particularly allowing long term-cultures and studies targeting specific pathways in IEC or immune cell compartments.

KEYWORDS:

3-D cultures; Intestinal epithelial cells; Intraepithelial lymphocytes; T cells

PMID:
25841547
PMCID:
PMC4451438
DOI:
10.1016/j.jim.2015.03.014
[Indexed for MEDLINE]
Free PMC Article

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