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J Infect Dis. 2015 Oct 1;212(7):1014-21. doi: 10.1093/infdis/jiv160. Epub 2015 Apr 3.

Pathogenic Role of Human Herpesvirus 6B Infection in Mesial Temporal Lobe Epilepsy.

Author information

1
Department of Pediatrics, Fujita Health University School of Medicine.
2
Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake.
3
National Epilepsy Center, Shizuoka Institute of Epilepsy and Neurological Disorder, Japan.

Abstract

BACKGROUND:

Human herpesvirus 6B (HHV-6B) is the causative agent for exanthem subitum. HHV-6B was associated with mesial temporal sclerosis (MTS), leading to mesial temporal lobe epilepsy (MTLE). In this study, we sought to elucidate the pathogenic role of HHV-6B in patients with MTLE.

METHODS:

Seventy-five intractable MTLE patients, including 52 MTS patients and 23 non-MTS patients, were enrolled in this study. Resected hippocampus, amygdala, and mixed samples of amygdala and uncus samples were examined by real-time polymerase chain reaction (PCR) and reverse-transcriptase PCR to detect viral DNA and messenger RNA (mRNA), respectively. Host gene expressions, including neural markers, were measured using the TaqMan Gene Expression Assay.

RESULTS:

Detection of HHV-6 DNA was higher in MTS patients than non-MTS patients (median/interquartile range: 19.1/0-89.2 vs 0.0/0.0-0.0 copies/µg DNA; P = .004). HHV-6B viral DNA was determined in 12/27 HHV-6 DNA-positive samples, and no HHV-6B mRNA were detected in all samples. In MTS patients, expression of monocyte chemotactic protein-1 (P = .029) and glial fibrillary acidic protein (P = .043) were significantly higher in the amygdala samples with HHV-6 DNA than those without viral DNA.

CONCLUSIONS:

This study suggests that HHV-6B may play an important role in the pathogenesis of MTS via modification of host gene expression.

KEYWORDS:

human herpesvirus 6; lobe; mesial temporal sclerosis

PMID:
25840441
DOI:
10.1093/infdis/jiv160
[Indexed for MEDLINE]

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