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Methods Cell Biol. 2015;127:349-86. doi: 10.1016/bs.mcb.2014.12.001. Epub 2015 Feb 14.

Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtii.

Author information

1
Department of Genetics, Washington University, St. Louis, MO, USA.
2
Department of Genetics, Washington University, St. Louis, MO, USA. Electronic address: dutcher@genetics.WustL.edu.

Abstract

Flagellar assembly requires intraflagellar transport of components from the cell body to the flagellar tip for assembly. The understanding of flagellar assembly has been aided by the ease of biochemistry and the availability of mutants in the unicellular green alga, Chlamydomonas reinhardtii. In this chapter, we discuss means to identify genes involved in these processes using forward and reverse genetics. In particular, the ease and low cost of whole genome sequencing (WGS) will help to make gene identification easier and promote the understanding of this important process.

KEYWORDS:

Artificial microRNAs; Conditional mutants; Flagellar transcriptome; Mapping; Mutagenesis; Suppressors; Whole genome sequencing

PMID:
25837400
PMCID:
PMC5777853
DOI:
10.1016/bs.mcb.2014.12.001
[Indexed for MEDLINE]
Free PMC Article

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