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Otolaryngol Head Neck Surg. 2015 Jul;153(1):130-6. doi: 10.1177/0194599815577784. Epub 2015 Apr 1.

Pediatric Autoimmune Neuropsychiatric Disorder Associated with Streptococcus Immunology: A Pilot Study.

Author information

1
Georgetown University School of Medicine, Washington, DC, USA Adw27@georgetown.edu.
2
Department of Otolaryngology-Head & Neck Surgery, Georgetown University Hospital, Washington, DC, USA.
3
Georgetown University Department of Biostatistics, Washington, DC, USA.
4
Georgetown University Department of Surgery, Washington, DC, USA.
5
Georgetown University School of Medicine, Washington, DC, USA.
6
Ear, Nose, & Throat Associates of Corpus Christi, Otolaryngology-Head and Neck 16 Surgery, Corpus Christi, Texas, USA.
7
Greater Washington Headache Center, Child Neurology, Bethesda, Maryland, USA.
8
Georgetown University School of Medicine, Washington, DC, USA Department of Otolaryngology-Head & Neck Surgery, Georgetown University Hospital, Washington, DC, USA.

Abstract

OBJECTIVE:

To elucidate specific cytokine and chemokine markers in patients diagnosed with pediatric autoimmune neuropsychiatric disorder associated with streptococcus (PANDAS).

STUDY DESIGN:

Prospective cohort study.

STUDY SETTING:

Academic university hospital.

METHODS:

Tonsil tissue was collected from 24 patients and organized into 3 groups: experimental PANDAS cohort (12 patients), group A beta hemolytic streptococcus control cohort (6 patients), and obstructive sleep apnea control cohort (6 patients). Each tissue sample was extracted with MSD Tris lysis buffer, and protein lysates were analyzed for human chemokines and cytokines by the Human Cytokine 30-Plex Assay on the Mesoscale System.

RESULTS:

We identified a significant difference in expression regarding the 8 following cytokines when comparing the experimental PANDAS, group A beta hemolytic streptococcus, and obstructive sleep apnea control cohorts: tumor necrosis factor-α and eotaxin-3. In addition, our group also identified a significant reduction in the expression of interleukin (IL)-8, interferon inducible protein-10, IL-17a, interferon-γ, IL-10, and IL-12 across the aforementioned groups.

CONCLUSIONS:

Patients diagnosed with PANDAS appear to maintain significantly different concentrations of cytokines when compared with patients afflicted by chronic group A beta hemolytic streptococcus infections and obstructive sleep apnea. As a result, one could potentially use the described characterization of immunologic markers as a basis for future mechanistic and epidemiological studies.

KEYWORDS:

immunology; pediatrics; tonsillectomy

PMID:
25832830
DOI:
10.1177/0194599815577784
[Indexed for MEDLINE]

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