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Sci Rep. 2015 Apr 1;5:9570. doi: 10.1038/srep09570.

A cost-effective RNA sequencing protocol for large-scale gene expression studies.

Author information

1
Morgridge Institute for Research, Madison, Wisconsin, United States of America.
2
1] Morgridge Institute for Research, Madison, Wisconsin, United States of America [2] Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America [3] Department of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara, Santa Barbara, California, United States of America.

Abstract

RNA sequencing has increasingly become an indispensable tool for biological research. While sequencing costs have fallen dramatically in recent years, the current cost of RNA sequencing, nonetheless, remains a barrier to even more widespread adoption. Here, we present a simple RNA sequencing protocol with substantially reduced costs. This protocol uses as little as 10 ng of total RNA, allows multiplex sequencing of up to 96 samples per lane, and is strand specific. Extensive validation using human embryonic stem cells showed high consistency between technical replicates at various multiplexing levels.

PMID:
25831155
PMCID:
PMC4381617
DOI:
10.1038/srep09570
[Indexed for MEDLINE]
Free PMC Article

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