Format

Send to

Choose Destination
J Equine Sci. 2015;26(1):25-9. doi: 10.1294/jes.26.25. Epub 2015 Mar 31.

Development of loop-mediated isothermal amplification methods for detecting Taylorella equigenitalis and Taylorella asinigenitalis.

Author information

1
Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
2
Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan ; Present address: Equine Department, Japan Racing Association, Tokyo 106-8401, Japan.

Abstract

Taylorella equigenitalis is a causative bacterium of contagious equine metritis (CEM), and Taylorella asinigenitalis is species belonging to genus Taylorella. The authors developed two loop-mediated isothermal amplification (LAMP) methods, Te-LAMP and Ta-LAMP, for detecting T. equigenitalis and T. asinigenitalis, respectively. Using experimentally spiked samples, Te-LAMP was as sensitive as a published semi-nested PCR method, and Ta-LAMP was more sensitive than conventional PCR. Multiplex LAMP worked well without nonspecific reactions, and the analytical sensitivities of multiplex LAMP in the spiked samples were almost equivalent to those of Te-LAMP and Ta-LAMP. Therefore, the LAMP methods are considered useful tools to detect T. equigenitalis and/or T. asinigenitalis, and preventive measures will be rapidly implemented if the occurrence of CEM is confirmed by the LAMP methods.

KEYWORDS:

Taylorella asinigenitalis; Taylorella equigenitalis; contagious equine metritis; loop-mediated isothermal amplification

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center