Format

Send to

Choose Destination
Nucleic Acids Res. 2015 Apr 30;43(8):4342-52. doi: 10.1093/nar/gkv185. Epub 2015 Mar 30.

Selective inhibition of miR-21 by phage display screened peptide.

Author information

1
Proteomics and Structural Biology Unit, Institute of Genomics and Integrative Biology, CSIR. Mathura Road, Delhi 110020, India.
2
Proteomics and Structural Biology Unit, Institute of Genomics and Integrative Biology, CSIR. Mathura Road, Delhi 110020, India Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi-110001, India.
3
Proteomics and Structural Biology Unit, Institute of Genomics and Integrative Biology, CSIR. Mathura Road, Delhi 110020, India Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi-110001, India National Chemical Laboratory, CSIR, Dr. Homi Bhabha Road, Pune 411008, India souvik@igib.res.in.

Abstract

miRNAs are nodal regulators of gene expression and deregulation of miRNAs is causally associated with different diseases, including cancer. Modulation of miRNA expression is thus of therapeutic importance. Small molecules are currently being explored for their potential to downregulate miRNAs. Peptides have shown to have better potency and selectivity toward their targets but their potential in targeting and modulating miRNAs remain unexplored. Herein, using phage display we found a very selective peptide against pre-miR-21. Interestingly, the peptide has the potential to downregulate miR-21, by binding to pre-miR-21 and hindering Dicer processing. It is selective towards miR-21 inside the cell. By antagonising miR-21 function, the peptide is able to increase the expression of its target proteins and thereby increase apoptosis and suppress cell proliferation, invasion and migration. This peptide can further be explored for its anti-cancer activity in vivo and may be even extended to clinical studies.

PMID:
25824952
PMCID:
PMC4417150
DOI:
10.1093/nar/gkv185
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center