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Int J Mol Sci. 2015 Mar 26;16(4):6855-67. doi: 10.3390/ijms16046855.

Resistance training regulates cardiac function through modulation of miRNA-214.

Author information

1
Laboratory of Biochemistry and Molecular Biology of the Exercise, School of Physical Education and Sport, University of Sao Paulo, Sao Paulo 05508-030, Brazil. stephanomelo@usp.br.
2
Laboratory of Molecular Physiology, Health Sciences Center, Federal University of Espírito Santo, Vitória 29043-900, Brazil. barauna2@gmail.com.
3
Department of Physical Education, Federal University of Viçosa, Viçosa, Minas Gerais 36570-900, Brazil. miguelefiufv@yahoo.com.br.
4
Laboratory of Biochemistry and Molecular Biology of the Exercise, School of Physical Education and Sport, University of Sao Paulo, Sao Paulo 05508-030, Brazil. luizbozi@hotmail.com.
5
Department of Physical Education, Federal University of Viçosa, Viçosa, Minas Gerais 36570-900, Brazil. lucasriosufv@yahoo.com.br.
6
Department of Physical Education, Federal University of Viçosa, Viçosa, Minas Gerais 36570-900, Brazil. anatali@ufv.br.
7
Laboratory of Biochemistry and Molecular Biology of the Exercise, School of Physical Education and Sport, University of Sao Paulo, Sao Paulo 05508-030, Brazil. edilamar@usp.br.

Abstract

AIMS:

To determine the effects of resistance training (RT) on the expression of microRNA (miRNA)-214 and its target in sarcoplasmic reticulum Ca2+-ATPase (SERCA2a), and on the morphological and mechanical properties of isolated left ventricular myocytes.

MAIN METHODS:

Male Wistar rats were divided into two groups (n = 7/group): Control (CO) or trained (TR). The exercise-training protocol consisted of: 4 × 12 bouts, 5×/week during 8 weeks, with 80% of one repetition maximum.

KEY FINDINGS:

RT increased the left ventricular myocyte width by 15% and volume by 12%, compared with control animals (p < 0.05). The time to half relaxation and time to peak were 8.4% and 4.4% lower, respectively, in cells from TR group as compared to CO group (p < 0.05). RT decreased miRNA-214 level by 18.5% while its target SERCA2a expression were 18.5% higher (p < 0.05).

SIGNIFICANCE:

Our findings showed that RT increases single left ventricular myocyte dimensions and also leads to faster cell contraction and relaxation. These mechanical adaptations may be related to the augmented expression of SERCA2a which, in turn, may be associated with the epigenetic modification of decreased miRNA-214 expression.

PMID:
25822872
PMCID:
PMC4424992
DOI:
10.3390/ijms16046855
[Indexed for MEDLINE]
Free PMC Article

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