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Nat Methods. 2015 May;12(5):407-10. doi: 10.1038/nmeth.3336. Epub 2015 Mar 30.

High-speed label-free functional photoacoustic microscopy of mouse brain in action.

Author information

1
Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri, USA.
2
Department of Electrical and Computer Engineering, Texas A&M University, College Station, Texas, USA.

Abstract

We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.

PMID:
25822799
PMCID:
PMC4428901
DOI:
10.1038/nmeth.3336
[Indexed for MEDLINE]
Free PMC Article

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