Format

Send to

Choose Destination
Clin Oral Investig. 2015 Nov;19(8):2075-89. doi: 10.1007/s00784-015-1443-2. Epub 2015 Mar 31.

Calcium silicate/calcium phosphate biphasic cements for vital pulp therapy: chemical-physical properties and human pulp cells response.

Author information

1
Dental School (Laboratory of Biomaterials and Oral Pathology), Department of Biomedical and NeuroMotor Sciences, University of Bologna, Bologna, Italy. mgiovanna.gandolfi@unibo.it.
2
Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples "Federico II", Napoli, Italy.
3
Dental School (Laboratory of Biomaterials and Oral Pathology), Department of Biomedical and NeuroMotor Sciences, University of Bologna, Bologna, Italy.

Abstract

OBJECTIVES:

The aim was to test the properties of experimental calcium silicate/calcium phosphate biphasic cements with hydraulic properties designed for vital pulp therapy as direct pulp cap and pulpotomy.

METHODS:

CaSi-αTCP and CaSi-DCDP were tested for ion-releasing ability, solubility, water sorption, porosity, ability to nucleate calcium phosphates, and odontoblastic differentiation—alkaline phosphatase (ALP) and osteocalcin (OCN) upregulation—of primary human dental pulp cells (HDPCs).

RESULTS:

The materials showed high Ca and OH release, high open pore volume and apparent porosity, and a pronounced ability to nucleate calcium phosphates on their surface. HDPCs treated with CaSi-αTCP showed a strong upregulation of ALP and OCN genes, namely a tenfold increase for OCN and a threefold increase for ALP compared to the control cells. Conversely, CaSi-DCDP induced a pronounced OCN gene upregulation but had no effect on ALP gene regulation.

CONCLUSIONS:

Both cements showed high biointeractivity (release of Ca and OH ions) correlated with their marked ability to nucleate calcium phosphates. CaSi-αTCP cement proved to be a potent inducer of ALP and OCN genes as characteristic markers of mineralization processes normally poorly expressed by HDPCs.

CLINICAL RELEVANCE:

Calcium silicate/calcium phosphate cements appear to be attractive new materials for vital pulp therapy as they may provide odontogenic/dentinogenic chemical signals for pulp regeneration and healing, and dentin formation in regenerative endodontics.

KEYWORDS:

Alkaline phosphatase; Biointeractivity; Calcium phosphate cements; Calcium release; Calcium silicate cements; Dentine regeneration; Genes activation; MTA; Osteocalcin; Porosity; Pulp cells; Pulp therapy; Regenerative dentistry; Solubility; pH

PMID:
25820989
DOI:
10.1007/s00784-015-1443-2
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center