Format

Send to

Choose Destination
J Orthop Res. 2015 Sep;33(9):1278-86. doi: 10.1002/jor.22906. Epub 2015 May 21.

Topographical investigation of changes in depth-wise proteoglycan distribution in rabbit femoral articular cartilage at 4 weeks after transection of the anterior cruciate ligament.

Author information

1
School of Medicine, University of Eastern Finland, Kuopio, FI-70211, Finland.
2
Institute of Biomedicine, Anatomy, University of Eastern Finland, Kuopio, FI-70211, Finland.
3
Department of Applied Physics, University of Eastern Finland, Kuopio, FI-70211, Finland.

Abstract

In this study, we explore topographical changes in proteoglycan distribution from femoral condylar cartilage in early osteoarthritis, acquired from both the lateral and medial condyles of anterior cruciate ligament transected (ACLT) and contralateral (CNTRL) rabbit knee joints, at 4 weeks post operation. Four sites across the cartilage surface in a parasagittal plane were defined across tissue sections taken from femoral condyles, and proteoglycan (PG) content was quantified using digital densitometry. The greatest depth-wise change in PG content due to an ACLT (compared to the CNTRL group) was observed anteriorly (site C) from the most weight-bearing location within the lateral compartment. In the medial compartment, the greatest change was observed in the most weight-bearing location (site B). The depth-wise changes in PG content were observed up to 48% and 28% depth from the tissue surface at these aforementioned sites, respectively (p < 0.05). The smallest depth-wise change in PG content was observed posteriorly (site A) from the most weight-bearing location within both femoral condyles (up to 20% and up to 5% depth from the tissue surface at lateral and medial compartments, respectively). This study gives further insight into how early cartilage deterioration progresses across the parasagittal plane of the femoral condyle.

KEYWORDS:

ACLT; cartilage; femoral condyle; proteoglycan content; rabbit

PMID:
25820864
DOI:
10.1002/jor.22906
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center