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Genetics. 2015 Jun;200(2):523-35. doi: 10.1534/genetics.115.176438. Epub 2015 Mar 27.

An Rtf2 Domain-Containing Protein Influences Pre-mRNA Splicing and Is Essential for Embryonic Development in Arabidopsis thaliana.

Author information

1
Institute of Plant and Microbial Biology, Nangang District, Taipei 115, Taiwan.
2
Institute of Plant and Microbial Biology, Nangang District, Taipei 115, Taiwan marjorimatzke@gate.sinica.edu.tw antoniusmatzke@gate.sinica.edu.tw.

Abstract

Alternative splicing is prevalent in plants, but little is known about its regulation in the context of developmental and signaling pathways. We describe here a new factor that influences pre-messengerRNA (mRNA) splicing and is essential for embryonic development in Arabidopsis thaliana. This factor was retrieved in a genetic screen that identified mutants impaired in expression of an alternatively spliced GFP reporter gene. In addition to the known spliceosomal component PRP8, the screen recovered Arabidopsis RTF2 (AtRTF2), a previously uncharacterized, evolutionarily conserved protein containing a replication termination factor 2 (Rtf2) domain. A homozygous null mutation in AtRTF2 is embryo lethal, indicating that AtRTF2 is an essential protein. Quantitative RT-PCR demonstrated that impaired expression of GFP in atrtf2 and prp8 mutants is due to inefficient splicing of the GFP pre-mRNA. A genome-wide analysis using RNA sequencing indicated that 13-16% of total introns are retained to a significant degree in atrtf2 mutants. Considering these results and previous suggestions that Rtf2 represents an ubiquitin-related domain, we discuss the possible role of AtRTF2 in ubiquitin-based regulation of pre-mRNA splicing.

KEYWORDS:

C2HC2 zinc finger; Rtf2 domain; alternative splicing; intron retention; ubiquitin ligase

PMID:
25819795
PMCID:
PMC4492377
DOI:
10.1534/genetics.115.176438
[Indexed for MEDLINE]
Free PMC Article

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