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Exp Cell Res. 2015 May 1;333(2):273-88. doi: 10.1016/j.yexcr.2015.03.015. Epub 2015 Mar 27.

Cell source-dependent in vivo immunosuppressive properties of mesenchymal stem cells derived from the bone marrow and synovial fluid of minipigs.

Author information

1
College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Gyeongnam, Republic of Korea.
2
Biomedical Research Institute, Gyeongsang National University Hospital, Jinju, Republic of Korea.
3
Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Gyeonggi, Republic of Korea.
4
Department of Internal Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea.
5
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada N1G 4S7.
6
College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Gyeongnam, Republic of Korea; Research Institute of Life Sciences, Gyeongsang National University, Jinju 660-701, Gyeongnam, Republic of Korea.
7
College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Gyeongnam, Republic of Korea; Research Institute of Life Sciences, Gyeongsang National University, Jinju 660-701, Gyeongnam, Republic of Korea. Electronic address: sllee@gnu.ac.kr.

Abstract

The in vitro differentiation and immunosuppressive capacity of mesenchymal stem cells (MSCs) derived from synovial fluid (SF-MSCs) and bone marrow extract (BM-MSCs) in an isogenic background of minipigs were comparatively analyzed in a collagen-induced arthritis (CIA) mouse model of rheumatoid arthritis (RA). The proliferation capacity and expression of pluripotent transcription factors (Oct3/4 and Sox2) were significantly (P<0.05) higher in SF-MSCs than in BM-MSCs. The differentiation capacity of SF-MSCs into adipocytes, osteocytes and neurocytes was significantly (P<0.05) lower than that of BM-MSCs, and the differentiation capacity of SF-MSCs into chondrocytes was significantly (P<0.05) higher than that of BM-MSCs. Systemic injection of BM- and SF-MSCs significantly (P<0.05) ameliorated the clinical symptoms of CIA mice, with SF-MSCs having significantly (P<0.05) higher clinical and histopathological recovery scores than BM-MSCs. Furthermore, the immunosuppressive properties of SF-MSCs in CIA mice were associated with increased levels of the anti-inflammatory cytokine interleukin (IL)-10, and decreased levels of the pro-inflammatory cytokine IL-1β and osteoclast-related sRANKL. In conclusion, SF-MSCs exhibited eminent pluripotency and differentiation capacity into chondrocytes, addition to substantial in vivo immunosuppressive capacity by elevating IL-10 and reducing IL-1β levels in CIA mice.

KEYWORDS:

Differentiation; Immunomodulation; Mesenchymal stem cell; Rheumatoid arthritis; Synovial fluid

PMID:
25819273
DOI:
10.1016/j.yexcr.2015.03.015
[Indexed for MEDLINE]
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