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Mol Cell. 2015 Apr 16;58(2):216-31. doi: 10.1016/j.molcel.2015.02.023. Epub 2015 Mar 26.

Widespread rearrangement of 3D chromatin organization underlies polycomb-mediated stress-induced silencing.

Author information

1
Department of Biology, Emory University, 1510 Clifton Road N.E., Atlanta, GA 30322, USA; Department of Biostatistics and Bioinformatics, Emory University, 1518 Clifton Road N.E., Atlanta, GA 30322, USA.
2
Department of Biology, Emory University, 1510 Clifton Road N.E., Atlanta, GA 30322, USA.
3
Department of Genetics, Geisel School of Medicine at Dartmouth, 609 Vail, Hanover, NH 03755, USA.
4
Department of Population Health, New York University School of Medicine, 650 First Avenue, New York, NY 10016, USA.
5
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD 20892, USA.
6
Department of Biostatistics and Bioinformatics, Emory University, 1518 Clifton Road N.E., Atlanta, GA 30322, USA.
7
Department of Biology, Emory University, 1510 Clifton Road N.E., Atlanta, GA 30322, USA. Electronic address: vcorces@emory.edu.

Abstract

Chromosomes of metazoan organisms are partitioned in the interphase nucleus into discrete topologically associating domains (TADs). Borders between TADs are formed in regions containing active genes and clusters of architectural protein binding sites. The transcription of most genes is repressed after temperature stress in Drosophila. Here we show that temperature stress induces relocalization of architectural proteins from TAD borders to inside TADs, and this is accompanied by a dramatic rearrangement in the 3D organization of the nucleus. TAD border strength declines, allowing for an increase in long-distance inter-TAD interactions. Similar but quantitatively weaker effects are observed upon inhibition of transcription or depletion of individual architectural proteins. Heat shock-induced inter-TAD interactions result in increased contacts among enhancers and promoters of silenced genes, which recruit Pc and form Pc bodies in the nucleolus. These results suggest that the TAD organization of metazoan genomes is plastic and can be reconfigured quickly.

PMID:
25818644
PMCID:
PMC4402144
DOI:
10.1016/j.molcel.2015.02.023
[Indexed for MEDLINE]
Free PMC Article

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