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Cell Rep. 2015 Mar 31;10(12):2083-95. doi: 10.1016/j.celrep.2015.02.065. Epub 2015 Mar 26.

Optical dissection of experience-dependent pre- and postsynaptic plasticity in the Drosophila brain.

Author information

1
Department of Molecular Neurobiology of Behavior, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University Göttingen, Julia-Lermontowa-Weg 3, 37077 Göttingen, Germany.
2
Department of Neuro- and Sensory Physiology, University of Göttingen Medical Center, Humboldtallee 23, 37073 Göttingen, Germany.
3
Department of Molecular Neurobiology of Behavior, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University Göttingen, Julia-Lermontowa-Weg 3, 37077 Göttingen, Germany. Electronic address: afiala@gwdg.de.

Abstract

Drosophila represents a key model organism for dissecting neuronal circuits that underlie innate and adaptive behavior. However, this task is limited by a lack of tools to monitor physiological parameters of spatially distributed, central synapses in identified neurons. We generated transgenic fly strains that express functional fluorescent reporters targeted to either pre- or postsynaptic compartments. Presynaptic Ca(2+) dynamics are monitored using synaptophysin-coupled GCaMP3, synaptic transmission is monitored using red fluorescent synaptophysin-pHTomato, and postsynaptic Ca(2+) dynamics are visualized using GCaMP3 fused with the postsynaptic matrix protein, dHomer. Using two-photon in vivo imaging of olfactory projection neurons, odor-evoked activity across populations of synapses is visualized in the antennal lobe and the mushroom body calyx. Prolonged odor exposure causes odor-specific and differential experience-dependent changes in pre- and postsynaptic activity at both levels of olfactory processing. The approach advances the physiological analysis of synaptic connections across defined groups of neurons in intact Drosophila.

PMID:
25818295
DOI:
10.1016/j.celrep.2015.02.065
[Indexed for MEDLINE]
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