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Anal Chim Acta. 2015 Apr 15;869:68-73. doi: 10.1016/j.aca.2015.02.010. Epub 2015 Feb 11.

Single-walled carbon nanotube based transparent immunosensor for detection of a prostate cancer biomarker osteopontin.

Author information

1
School of Mechanical and Nuclear Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798, Republic of Korea.
2
School of Mechanical and Nuclear Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798, Republic of Korea; Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798, Republic of Korea; School of Materials Science and Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798, Republic of Korea. Electronic address: jjang@unist.ac.kr.

Abstract

Osteopontin (OPN) is involved in almost all steps of cancer development, and it is being investigated as a potential biomarker for a diagnosis and prognosis of prostate cancer. Here, we report a label-free, highly sensitive and transparent immunosensor based on single-walled carbon nanotubes (SWCNTs) for detection of OPN. A high density of COOH functionalized SWCNTs was deposited between two gold/indium tin oxide electrodes on a glass substrate by dielectrophoresis. Monoclonal antibodies specific to OPN were covalently immobilized on the SWCNTs. Relative resistance change of the immunosensors was measured as the concentration of OPN in phosphate buffer saline (PBS) and human serum was varied from 1 pg mL(-1) to 1 μg mL(-1) for different channel lengths of 2, 5, and 10 μm, showing a highly linear and reproducible behavior (R(2)>97%). These immunosensors were also specific to OPN against another test protein, bovine serum albumin, PBS and human serum, showing that a limit of detection for OPN was 0.3 pg mL(-1). This highly sensitive and transparent immunosensor has a great potential as a simple point-of-care test kit for various protein biomarkers.

KEYWORDS:

Cancer; Carbon nanotubes; Dielectrophoresis; Field effect transistor sensors; Osteopontin; Transparent sensor

PMID:
25818141
DOI:
10.1016/j.aca.2015.02.010
[Indexed for MEDLINE]

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