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Carbohydr Polym. 2015 May 20;122:93-100. doi: 10.1016/j.carbpol.2014.12.032. Epub 2014 Dec 31.

Purification, characterization and neuroprotective effects of a polysaccharide from Gynostemma pentaphyllum.

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Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China.
Department of Neurology, Xi'an Central Hospital, Xi'an 710003, China.
Department of Neurosurgery, Shangluo Central Hospital, Shangluo 726000, China.
Emergency Department, Xi'an Children's Hospital, Xi'an 710003, China. Electronic address:


In the present study, we investigated the neuroprotective effects of GPP1, a purified polysaccharide from Gynostemma pentaphyllum, on Aβ (25-35)-induced neuronal toxicity and explore its potential mechanisms in PC12 cells. The results showed pretreatment with GPP1 prior to Aβ (25-35) exposure significantly protected PC12 cells from Aβ (25-35)-induced cell death, lactate dehydrogenase (LDH) release, DNA damage, intracellular free calcium concentration ([Ca(2+)]i) influx, mitochondrial dysfunction and mitochondrial cytochrome c (Cyt-C) release. In addition, pretreatment with GPP1 also protected PC12 cells against Aβ (25-35)-induced the accumulation of reactive oxygen species (ROS) and lipid peroxidation product MDA, the reduction in glutathione (GSH) level and superoxide dismutase (SOD) activity, the increased Bax/Bcl-2 protein expression ratio and caspase-3 activation. Our findings suggest that GPP1 exerts a neuroprotective effect against Aβ (25-35)-induced neurotoxicity in PC12 cells, at least in part, via inhibiting oxidative stress and suppressing the mitochondrial apoptotic pathway.


Antioxidant; Apoptosis; Gynostemma pentaphyllum; Isolation; Polysaccahride

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