Format

Send to

Choose Destination
Carbohydr Polym. 2015 May 20;122:93-100. doi: 10.1016/j.carbpol.2014.12.032. Epub 2014 Dec 31.

Purification, characterization and neuroprotective effects of a polysaccharide from Gynostemma pentaphyllum.

Author information

1
Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China.
2
Department of Neurology, Xi'an Central Hospital, Xi'an 710003, China.
3
Department of Neurosurgery, Shangluo Central Hospital, Shangluo 726000, China.
4
Emergency Department, Xi'an Children's Hospital, Xi'an 710003, China. Electronic address: jinlilei_apl@126.com.

Abstract

In the present study, we investigated the neuroprotective effects of GPP1, a purified polysaccharide from Gynostemma pentaphyllum, on Aβ (25-35)-induced neuronal toxicity and explore its potential mechanisms in PC12 cells. The results showed pretreatment with GPP1 prior to Aβ (25-35) exposure significantly protected PC12 cells from Aβ (25-35)-induced cell death, lactate dehydrogenase (LDH) release, DNA damage, intracellular free calcium concentration ([Ca(2+)]i) influx, mitochondrial dysfunction and mitochondrial cytochrome c (Cyt-C) release. In addition, pretreatment with GPP1 also protected PC12 cells against Aβ (25-35)-induced the accumulation of reactive oxygen species (ROS) and lipid peroxidation product MDA, the reduction in glutathione (GSH) level and superoxide dismutase (SOD) activity, the increased Bax/Bcl-2 protein expression ratio and caspase-3 activation. Our findings suggest that GPP1 exerts a neuroprotective effect against Aβ (25-35)-induced neurotoxicity in PC12 cells, at least in part, via inhibiting oxidative stress and suppressing the mitochondrial apoptotic pathway.

KEYWORDS:

Antioxidant; Apoptosis; Gynostemma pentaphyllum; Isolation; Polysaccahride

PMID:
25817647
DOI:
10.1016/j.carbpol.2014.12.032
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center