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Anal Biochem. 2015 Jun 15;479:60-2. doi: 10.1016/j.ab.2015.03.023. Epub 2015 Mar 27.

Combining qualitative and quantitative imaging evaluation for the assessment of genomic DNA integrity: The SPIDIA experience.

Author information

1
Unit of Medical Statistics, Biometry, and Bioinformatics, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy.
2
Department of Experimental and Clinical Biomedical Sciences, University of Florence, 50139 Florence, Italy.
3
Qiagen GmbH, 40724 Hilden, Germany.
4
Unit of Medical Statistics, Biometry, and Bioinformatics, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy. Electronic address: paolo.verderio@istitutotumori.mi.it.

Abstract

In this note, we present an ad hoc procedure that combines qualitative (visual evaluation) and quantitative (ImageJ software) evaluations of Pulsed-Field Gel Electrophoresis (PFGE) images to assess the genomic DNA (gDNA) integrity of analyzed samples. This procedure could be suitable for the analysis of a large number of images by taking into consideration both the expertise of researchers and the objectiveness of the software. We applied this procedure on the first SPIDIA DNA External Quality Assessment (EQA) samples. Results show that the classification obtained by this ad hoc procedure allows a more accurate evaluation of gDNA integrity with respect to a single approach.

KEYWORDS:

External Quality Assessment (EQA); ImageJ software; PFGE; gDNA integrity

PMID:
25817220
DOI:
10.1016/j.ab.2015.03.023
[Indexed for MEDLINE]

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