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Oxid Med Cell Longev. 2015;2015:792846. doi: 10.1155/2015/792846. Epub 2015 Feb 25.

Role of circulating miRNAs as biomarkers in idiopathic pulmonary arterial hypertension: possible relevance of miR-23a.

Author information

1
Universidad Catolica de Valencia, Guillem de Castro Street 106, 46003 Valencia, Spain.
2
Department of Pathology, Faculty of Medicine and Odontology, University of Valencia, Blasco Ibáñez Avenue 15, 46010 Valencia, Spain ; Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO), Cataluña Avenue 21, 46020 Valencia, Spain.
3
Hospital General Universitario de Valencia, Tres Cruces Avenue, 46014 Valencia, Spain ; Department of Medicine, Faculty of Medicine and Odontology, University of Valencia, Blasco Ibáñez Avenue 15, 46010 Valencia, Spain.
4
Hospital Universitario Doctor Peset, Gaspar Aguilar Street 90, 46017 Valencia, Spain.
5
Department of Pathology, Faculty of Medicine and Odontology, University of Valencia, Blasco Ibáñez Avenue 15, 46010 Valencia, Spain ; Fundación para la Investigación del Hospital Clínico de la Comunidad Valenciana (INCLIVA), Blasco Ibañez Avenue 15, 46010 Valencia, Spain.
6
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Carretera Soller Km 12, Bunyola, Mallorca, 07110 Illes Balears, Spain ; Department of Pharmacology, Faculty of Medicine and Odontology, University of Valencia, Blasco Ibáñez Avenue 15, 46010 Valencia, Spain ; Fundación de Investigación Hospital General Universitario de Valencia, Tres Cruces Avenue 3, 46014 Valencia, Spain.
7
Department of Pathology, Faculty of Medicine and Odontology, University of Valencia, Blasco Ibáñez Avenue 15, 46010 Valencia, Spain ; Fundación para la Investigación del Hospital Clínico de la Comunidad Valenciana (INCLIVA), Blasco Ibañez Avenue 15, 46010 Valencia, Spain ; Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Carretera Soller Km 12, Bunyola, Mallorca, 07110 Illes Balears, Spain.

Abstract

Idiopathic pulmonary hypertension (IPAH) is a rare disease characterized by a progressive increase in pulmonary vascular resistance leading to heart failure. MicroRNAs (miRNAs) are small noncoding RNAs that control the expression of genes, including some involved in the progression of IPAH, as studied in animals and lung tissue. These molecules circulate freely in the blood and their expression is associated with the progression of different vascular pathologies. Here, we studied the expression profile of circulating miRNAs in 12 well-characterized IPAH patients using microarrays. We found significant changes in 61 miRNAs, of which the expression of miR23a was correlated with the patients' pulmonary function. We also studied the expression profile of circulating messenger RNA (mRNAs) and found that miR23a controlled 17% of the significantly changed mRNA, including PGC1α, which was recently associated with the progression of IPAH. Finally we found that silencing of miR23a resulted in an increase of the expression of PGC1α, as well as in its well-known regulated genes CYC, SOD, NRF2, and HO1. The results point to the utility of circulating miRNA expression as a biomarker of disease progression.

PMID:
25815108
PMCID:
PMC4357130
DOI:
10.1155/2015/792846
[Indexed for MEDLINE]
Free PMC Article

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