Format

Send to

Choose Destination
Nat Commun. 2015 Mar 27;6:6560. doi: 10.1038/ncomms7560.

Capturing the cloud of diversity reveals complexity and heterogeneity of MRSA carriage, infection and transmission.

Author information

1
Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, UK.
2
Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
3
Wellcome Trust Sanger Institute, Hinxton CB10 15A, UK.
4
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht 3584 CL, The Netherlands.
5
Department of Clinical Medicine, University of Cambridge, Cambridge CB2 0QQ, UK.

Abstract

Genome sequencing is revolutionizing clinical microbiology and our understanding of infectious diseases. Previous studies have largely relied on the sequencing of a single isolate from each individual. However, it is not clear what degree of bacterial diversity exists within, and is transmitted between individuals. Understanding this 'cloud of diversity' is key to accurate identification of transmission pathways. Here, we report the deep sequencing of methicillin-resistant Staphylococcus aureus among staff and animal patients involved in a transmission network at a veterinary hospital. We demonstrate considerable within-host diversity and that within-host diversity may rise and fall over time. Isolates from invasive disease contained multiple mutations in the same genes, including inactivation of a global regulator of virulence and changes in phage copy number. This study highlights the need for sequencing of multiple isolates from individuals to gain an accurate picture of transmission networks and to further understand the basis of pathogenesis.

PMID:
25814293
PMCID:
PMC4389252
DOI:
10.1038/ncomms7560
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center