Format

Send to

Choose Destination
PLoS One. 2015 Mar 26;10(3):e0121290. doi: 10.1371/journal.pone.0121290. eCollection 2015.

Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.

Author information

1
Department of Medicine, San Francisco VA Medical Center, San Francisco, California, United States of America; Department of Medicine, University of California, San Francisco (UCSF), San Francisco, California, United States of America.
2
Department of Medicine, University of California, San Francisco (UCSF), San Francisco, California, United States of America.
3
School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.
4
Department of Microbiology, University of Minnesota, Minneapolis, Minnesota, United States of America.

Abstract

Gastrointestinal T lymphocytes are critical for mucosal immunity and HIV pathogenesis, yet little is known about normal T cell numbers and phenotypes in different regions of the gut, or the degree to which ART can restore levels to those of HIV-uninfected individuals. To investigate these questions, we measured T cell frequencies and markers of memory, activation, anergy, and homing in the blood, ileum, and rectum of HIV- and ART-suppressed HIV+ adults. In HIV- individuals, T cell frequencies and phenotypes differed significantly between sites. Compared to HIV- adults, HIV+ adults had lower absolute CD4+T cell counts in the ileal lamina propria and lower relative CD4+T cell counts in the blood and ileum. In the gut, HIV+ adults had a higher proportion of CD38+ CD4+T cells, a lower proportion of terminally-differentiated effector cells, and, in the rectum, a higher proportion of CTLA-4+ CD4+T cells. In HIV+ individuals, relative CD4+T cell numbers in the ileum correlated with the proportion of CTLA-4+ CD4+T cells, whereas in the rectum, they tended to correlate with the proportion of circulating CD4+T cells expressing α4β7 or CCR6. Mechanisms of T cell reconstitution may differ throughout the gut, with homing contributing more in the rectum while ileal reconstitution is associated with mucosal CD4+T cell anergy.

PMID:
25811360
PMCID:
PMC4374729
DOI:
10.1371/journal.pone.0121290
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center