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Dis Esophagus. 2016 Oct;29(7):843-847. doi: 10.1111/dote.12346. Epub 2015 Mar 23.

Intestinal epithelial culture under an air-liquid interface: a tool for studying human and mouse esophagi.

Author information

1
Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
2
Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Japan.
3
Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan.
4
Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Japan. mmori@gesurg.med.osaka-u.ac.jp.

Abstract

This study investigated whether an intestinal epithelial culture method can be applied to mouse and human esophageal cultures. The esophagi harvested from 1-day-old mice and adult humans were maintained in collagen gels. A commercially available culture medium for human embryonic stem cells was used for the human esophageal culture. We discovered that the intestinal epithelial culture method can be successfully applied to both mouse and human esophageal cultures. The long-term cultured esophageal organoids were rod-like luminal structures lined with myofibroblasts. We discovered that regeneration of the esophageal mucosal surface can be almost completely achieved in vitro, and the advantage of this method is that organoid cultures may be generated using host-derived fibroblasts as a niche. This method is a promising tool for mouse and human research in intestinal biology, carcinogenesis, and regenerative medicine.

KEYWORDS:

esophageal culture; keratin; organ culture

PMID:
25809505
DOI:
10.1111/dote.12346
[Indexed for MEDLINE]

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